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L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropionic acid trifluoroacetic salt

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Availability: In stock

Catalog: ASIS-0014

Formal Name: L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropionic acid trifluoroacetic salt

Produced By: AsisChem, Inc.

Related Files
pdfASIS-0014-Technical Product Information (532.82 KB )
pdfASIS-0014 Safety Date Sheet (254.1 KB )

Biological Description

L-ANAP is a fluorescent unnatural amino acid which enables the fluorescent labeling of any protein by changing a single codon to incorporate this amino acid at any permissive site. It has utility in FRET assays and biophysical studies. It has been successfully incorporated into proteins in Xenopus oocytes, yeast (Lee, 2009), and mammalian cells (CHO, HEK293) (Chatterjee, 2013). It is ideal for protein conformation and post-translational modification experiments. Additionally, L-ANAP exhibits a spectral shift in emission dependent upon surrounding polarity making it uniquely capable of indicating environmental changes.L-ANAP has an excitation maxima of 360nm, emission maxima 490nm in water with shifts as far as 420nm. Extinction coefficient 17,500 cm-1M-1. Non-toxic in mammalian cells at concentrations as high as 500 uM (Chatterjee, 2013).The L-ANAP-OMe variant (ASIS-0146) has increased solubility compared to the related product, L-ANAP TFA (ASIS-0014).Incorporation of L-ANAP requires the expression of a modified tRNA/synthetase pair derived from E. coli (Chatterjee, 2013). A mammalian expression plasmid containing the amber suppressor tRNA and the synthetase AnapRS is available from Addgene (www.addgene.org/48696).

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Additional Information

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Catalog ASIS-0014
Formal Name L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropionic acid trifluoroacetic salt
CAS Number 1313516-26-5
Molecular Weight 272.00
Formula C15 H16 N2 O3
Lead Time 1
LogP 2.639499903
Purity 95
SMILES CC(=O)C1=CC2=CC=C(C=C2C=C1)NC[C@@H](N)C(O)=O |&1:15|
Solubility and Handling Partially soluble in DMSO and EtOH. Dissolve in an aliquot of DMSO, add it to water or saline buffer to form 10 µM L-ANAP TFA in 0.2-1.0 % water DMSO solution. Another option : dissolve to 1 mM in water with NaOH as needed
inchikey XKZCXMNMUMGDJG-AWEZNQCLSA-N
TPSA 92.41999817
HBD 3
HBA 5

Details

Wang, Q. and L. Wang, New methods enabling efficient incorporation of unnatural amino acids in yeast. J Am Chem Soc, 2008. 130(19): p. 6066-7. (Abstract)(Abstract)

Chatterjee, A., et al., A genetically encoded fluorescent probe in mammalian cells. J Am Chem Soc, 2013. 135(34): p. 12540-3. (Abstract)(Abstract)

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You're reviewing: L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropionic acid trifluoroacetic salt

Product Details

Additional Information

Quantities Please contact us for larger or different quantities
Catalog ASIS-0014
Formal Name L-3-(6-acetylnaphthalen-2-ylamino)-2-aminopropionic acid trifluoroacetic salt
CAS Number 1313516-26-5
Molecular Weight 272.00
Formula C15 H16 N2 O3
Lead Time 1
LogP 2.639499903
Purity 95
SMILES CC(=O)C1=CC2=CC=C(C=C2C=C1)NC[C@@H](N)C(O)=O |&1:15|
Solubility and Handling Partially soluble in DMSO and EtOH. Dissolve in an aliquot of DMSO, add it to water or saline buffer to form 10 µM L-ANAP TFA in 0.2-1.0 % water DMSO solution. Another option : dissolve to 1 mM in water with NaOH as needed
inchikey XKZCXMNMUMGDJG-AWEZNQCLSA-N
TPSA 92.41999817
HBD 3
HBA 5
References

Details

Wang, Q. and L. Wang, New methods enabling efficient incorporation of unnatural amino acids in yeast. J Am Chem Soc, 2008. 130(19): p. 6066-7. (Abstract)(Abstract)

Chatterjee, A., et al., A genetically encoded fluorescent probe in mammalian cells. J Am Chem Soc, 2013. 135(34): p. 12540-3. (Abstract)(Abstract)

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