Formal Name: 2-amino-3-(4-(benzoyl-2,3,4,5,6-d5)phenyl-2,3,5,6-d4)propanoic-3,3-d2 acid hydrochloride
Produced By: AsisChem, Inc.
p-Benzoyl-DL-Phenylalanine-D11 (pBpF-D11), ASIS-0076, is a racemic, deuterated form of the unnatural amino acid (UAA) p-benzyoyl-phenylalanine. pBpF is a genetically encodable UAA which can be used to induce site specific photo crosslinking of any protein with an amber suppressor mutation technique (Rust, Subramanian et al. 2014. This deuterated form can be used with mass spectroscopy experiments. Site specific incorporation of pBpF or the deuterated form relies upon an orthogonal tRNA and amino acyl tRNA synthetase derived from a Bacillus stearothermophilus amber suppressor tRNA and amino acyl synthetase found in the pEVOL-pBpF plasmid (Rust, Subramanian et al. 2014). Published experiments using the non-deuterated form of pBpF cite a working concentration of 0.25-0.5 mM in E. coli C43(DE3). pBpF has also been used in mammalian cell culture (Bray, Mouledous et al. 2014, Valentin-Hansen, Park et al. 2014, ).